Simultaneous detection of nonpolar and polar compounds by heat-assisted laser ablation electrospray ionization mass spectrometry.

Solvent gradient electrospray for laser ablation electrospray ionization mass spectrometry.

Most electrospray based ambient ionization techniques, e.g., laser ablation electrospray ionization (LAESI), utilize a fixed spray solution composition. Complex samples often contain compounds of different polarity that exhibit a wide range of solubilities in the electrospray solvent. Thus, the fixed spray solution composition limits the molecular coverage of these approaches. Two-barrel theta ...

Laser ablation atmospheric pressure photoionization mass spectrometry imaging of phytochemicals from sage leaves.

RATIONALE Despite fast advances in ambient mass spectrometry imaging (MSI), the study of neutral and nonpolar compounds directly from biological matrices remains challenging. In this contribution, we explore the feasibility of laser ablation atmospheric pressure photoionization (LAAPPI) for MSI of phytochemicals in sage (Salvia officinalis) leaves. METHODS Sage leaves were studied by LAAPPI-t...

Mass spectrometry sampling under ambient conditions with desorption electrospray ionization.

A new method of desorption ionization is described and applied to the ionization of various compounds, including peptides and proteins present on metal, polymer, and mineral surfaces. Desorption electrospray ionization (DESI) is carried out by directing electrosprayed charged droplets and ions of solvent onto the surface to be analyzed. The impact of the charged particles on the surface produce...

Observation of subcellular metabolite gradients in single cells by laser ablation electrospray ionization mass spectrometry.

Toward single-cell analysis by plume collimation in laser ablation electrospray ionization mass spectrometry.

Ambient ionization methods for mass spectrometry have enabled the in situ and in vivo analysis of biological tissues and cells. When an etched optical fiber is used to deliver laser energy to a sample in laser ablation electrospray ionization (LAESI) mass spectrometry, the analysis of large single cells becomes possible. However, because in this arrangement the ablation plume expands in three d...